Journal: PLoS ONE

Article Title: Unique Cell Type-Specific Junctional Complexes in Vascular Endothelium of Human and Rat Liver Sinusoids

doi: 10.1371/journal.pone.0034206

Figure Lengend Snippet: Primary antibodies used in the study.

Article Snippet: anti-LYVE-1 polyclonal rabbit Ig , 103-PA50 , RELIATech.

Techniques: Transduction

Journal: PLoS ONE

Article Title: Unique Cell Type-Specific Junctional Complexes in Vascular Endothelium of Human and Rat Liver Sinusoids

doi: 10.1371/journal.pone.0034206

Figure Lengend Snippet: (A) Immunofluorescent co-staining of human liver cryosections with anti-VE-cadherin (green) and anti-CD32b (red) antibodies. (B) Immunofluorescent co-staining of rat liver cryosections with anti-VE-cadherin (green) and anti-LYVE-1 (red) antibodies. (C) Immunofluorescent co-staining of isolated rat LSECs with anti-VE-cadherin (green) and anti-Stabilin-2 (red) antibodies. Toto3 (blue) was used to counterstain the cell nuclei. Images were acquired using laser scanning confocal microscopy. Bars 11.9 µm (A, B), 14.14 µm (C). (D) Reverse transcriptase-PCR with mRNA isolated from rat hepatoma McA-RH7777 cell line (1), freshly isolated rat LMECs (2), and freshly isolated rat LSECs (3). Primers specific for VE-cadherin or β-actin were used.

Article Snippet: anti-LYVE-1 polyclonal rabbit Ig , 103-PA50 , RELIATech.

Techniques: Staining, Isolation, Confocal Microscopy

Journal: PLoS ONE

Article Title: Unique Cell Type-Specific Junctional Complexes in Vascular Endothelium of Human and Rat Liver Sinusoids

doi: 10.1371/journal.pone.0034206

Figure Lengend Snippet: (A, B) Immunofluorescent co-staining of rat liver cryosections with anti-E-cadherin (A, green) or anti-N-cadherin (B, green) and anti-LYVE-1 (A, B, red) antibodies. (C, D) Immunofluorescent co-staining of human liver cryosections with anti-E-cadherin (C, green) or anti-N-cadherin (D, green) and anti-VE-cadherin (C, D, red) antibodies. Images were acquired using laser scanning confocal microscopy. Bars 14.14 µm (A, B, D), 11.9 µm (C). (E) Reverse transcriptase-PCR with mRNA of freshly isolated rat LSECs. Primers specific for VE-cadherin (1), E-cadherin (2), N-cadherin (3) or β-actin (4) were used.

Article Snippet: anti-LYVE-1 polyclonal rabbit Ig , 103-PA50 , RELIATech.

Techniques: Staining, Confocal Microscopy, Isolation

Journal: PLoS ONE

Article Title: Unique Cell Type-Specific Junctional Complexes in Vascular Endothelium of Human and Rat Liver Sinusoids

doi: 10.1371/journal.pone.0034206

Figure Lengend Snippet: (A-D) Immunofluorescent co-staining of rat liver cryosections with anti-α-Catenin (A, green), anti-β-Catenin (B, green), anti-p120-Catenin (C, green), anti-Plakoglobin (D, green), anti-VE -cadherin (A-D, red), anti-Stabilin-2 (A, blue), and anti-LYVE-1 (B-D, blue) antibodies. Images were acquired using laser scanning confocal microscopy. Bars 11.9 µm.

Article Snippet: anti-LYVE-1 polyclonal rabbit Ig , 103-PA50 , RELIATech.

Techniques: Staining, Confocal Microscopy

Journal: PLoS ONE

Article Title: Unique Cell Type-Specific Junctional Complexes in Vascular Endothelium of Human and Rat Liver Sinusoids

doi: 10.1371/journal.pone.0034206

Figure Lengend Snippet: (A, C) Immunofluorescent co-staining of rat liver cryosections with anti-Occludin (A, green), anti-Claudin-5 (C, green), and anti-LYVE-1 (A, C, blue) antibodies. (E) Immunofluorescent staining of a liver sample obtained from the patient 2 with anti-Occludin (green) antibody; BD – bile ducts, S – liver sinusoids. (F, G) Immunofluorescent co-staining of liver samples obtained from the patients 6 (F) and 4 (G) with anti-VE-cadherin (F, G, green), anti-CD32b (F, G, red), and anti-Occludin (F, G, blue) antibodies. Images were acquired using laser scanning confocal microscopy. Bars 11.9 µm (A, C), 47.62 µm (E, F), 14.14 µm (G). (B, D) Quantitative reverse transcriptase-PCR with mRNA isolated from rat LSECs and rat LMECs (n indicates the number of samples analyzed, error bars represent SEM). Primers specific for Occludin (B), Claudin-5 (D), and β-Actin as normalizer were used.

Article Snippet: anti-LYVE-1 polyclonal rabbit Ig , 103-PA50 , RELIATech.

Techniques: Staining, Confocal Microscopy, Isolation

Journal: International Journal of Breast Cancer

Article Title: Lymphangiogenesis and Axillary Lymph Node Metastases Correlated with VEGF-C Expression in Two Immunocompetent Mouse Mammary Carcinoma Models

doi: 10.4061/2011/867152

Figure Lengend Snippet: Immunohistochemistry of LYVE-1 in BJMC338 tumors (a, c, e, and g) and BJMC3879 tumors (b, d, f, and h) at 4 weeks (a and b), 6 weeks (c and d), 8 weeks (e and f), and 10 weeks postinoculation (g and h). Note the upregulation of LYVE-1 in BJMC3879 tumors.

Article Snippet: To visualize lymphatic vessels, tumors were stained with primary antibodies to the lymphatic-specific marker, lymphatic vessel endothelial hyaluronan receptor-1 (LYVE-1) (rabbit polyclonal, Acris Antibodies GmbH, Hiddenhausen, Germany).

Techniques: Immunohistochemistry